@article {921211,
	title = {Tuatara (Sphenodon) genomics: BAC library construction, sequence survey, and application to the DMRT gene family},
	journal = {J Hered},
	volume = {97},
	number = {6},
	year = {2006},
	note = {<p>0022-1503 (Print)Journal ArticleResearch Support, N.I.H., ExtramuralResearch Support, U.S. Gov{\textquoteright}t, Non-P.H.S.</p>},
	month = {Nov-Dec},
	pages = {541-8},
	abstract = {<p>The tuatara (Sphenodon punctatus) is of "extraordinary biological interest" as the most distinctive surviving reptilian lineage (Rhyncocephalia) in the world. To provide a genomic resource for an understanding of genome evolution in reptiles, and as part of a larger project to produce genomic resources for various reptiles (evogen.jgi.doe.gov/second_levels/BACs/our_libraries.html), a large-insert bacterial artificial chromosome (BAC) library from a male tuatara was constructed. The library consists of 215 424 individual clones whose average insert size was empirically determined to be 145 kb, yielding a genomic coverage of approximately 6.3x. A BAC-end sequencing analysis of 121 420 bp of sequence revealed a genomic GC content of 46.8\%, among the highest observed thus far for vertebrates, and identified several short interspersed repetitive elements (mammalian interspersed repeat-type repeats) and long interspersed repetitive elements, including chicken repeat 1 element. Finally, as a quality control measure the arrayed library was screened with probes corresponding to 2 conserved noncoding regions of the candidate sex-determining gene DMRT1 and the DM domain of the related DMRT2 gene. A deep coverage contig spanning nearly 300 kb was generated, supporting the deep coverage and utility of the library for exploring tuatara genomics.</p>},
	keywords = {*Chromosomes, Artificial, Bacterial, *Multigene Family, Animals, Bayes Theorem, Gene Library, genome, Genomics/methods, Male, Phylogeny, Reptiles/*genetics, Transcription Factors/classification/*genetics},
	author = {Wang, Z. and Miyake, T. and Edwards, S. V. and Amemiya, C. T.}
}
