@article {507551, title = {Exploration of phylogenetic data using a global sequence analysis method}, journal = {BMC Evolutionary Biology}, volume = {5}, year = {2005}, pages = {63}, abstract = {

Background

Molecular phylogenetic methods are based on alignments of nucleic or peptidic sequences. The tremendous increase in molecular data permits phylogenetic analyses of very long sequences and of many species, but also requires methods to help manage large datasets.

Results

Here we explore the phylogenetic signal present in molecular data by genomic signatures, defined as the set of frequencies of short oligonucleotides present in DNA sequences. Although violating many of the standard assumptions of traditional phylogenetic analyses {\textendash} in particular explicit statements of homology inherent in character matrices {\textendash} the use of the signature does permit the analysis of very long sequences, even those that are unalignable, and is therefore most useful in cases where alignment is questionable. We compare the results obtained by traditional phylogenetic methods to those inferred by the signature method for two genes: RAG1, which is easily alignable, and 18S RNA, where alignments are often ambiguous for some regions. We also apply this method to a multigene data set of 33 genes for 9 bacteria and one archea species as well as to the whole genome of a set of 16 γ-proteobacteria. In addition to delivering phylogenetic results comparable to traditional methods, the comparison of signatures for the sequences involved in the bacterial example identified putative candidates for horizontal gene transfers.

Conclusion

The signature method is therefore a fast tool for exploring phylogenetic data, providing not only a pretreatment for discovering new sequence relationships, but also for identifying cases of sequence evolution that could confound traditional phylogenetic analysis.

}, url = {http://bmcevolbiol.biomedcentral.com/articles/10.1186/1471-2148-5-63}, author = {Chapus, C and C Dufraigne and S Edwards and Giron, A and Fertil, B and P Deschavanna} }